九州影院

For details on the confocal microscope see the tab above under specialised equipment.

• Email听confocal@canterbury.ac.nz

We offer a range of high-quality sequencing and genotyping services including Sanger sequencing, microsatellite genotyping, and AFLP genotyping using an Applied Biosystems 3130xl Genetic Analyzer.

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External customers

Sequencing: We offer two options.听

• Full service 鈥� you provide purified PCR products and PCR primers, we do the rest 鈥� $15 per sample.
• Capillary separation only 鈥� you provided purified sequencing products, we do the rest 鈥� $10 per sample.

Genotyping

• Capillary separation only - you supply the internal size standard, you prepare the plate, and we take care of the rest - $10 per sample.

There is a 16-sample minimum. If you submit fewer than 16 samples they will not be processed until we have obtained additional samples from other clients.

Email us on听dnaseq@exchange.canterbury.ac.nz听to make a booking. Once you have a booking, download the relevant request form and email it to us, as well as including a hard copy with your samples. 听Turnaround time is usually 24 hours.

• Sequencing external request form (Word, 187KB)
• Genotyping external request form (Word, 148KB)

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Sample preparation

For full sequencing service we require purified PCR products and PCR primers.

• PCR products must be free of residual salts, chelating agents (eg, EDTA), proteins, solvents, and buffers. Several PCR purification methods are available. Please choose the product that best suits your needs.
• PCR products must be quantified prior to shipment (by eye, Nanodrop, etc). Please provide concentrations in ng/碌l.
• Please provide PCR primer(s) at a concentration of 3.2 碌M.

For capillary separation only service we require: purified sequencing products

• Sequencing products must be free of unincorporated fluorescent nucleotides. Several sequencing purification methods are available. Please choose the product that best suits your needs.

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Genotyping service

If you require an internal size standard, we offer GeneScan 500 LIZ for microsatellite genotyping and MapMarker 1000 ROX for AFLP genotyping.
Please contact us for advice regarding plate preparation.

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Internal customers

Sequencing service

We offer two options:

• Full service - you provide purified PCR products and PCR primers, we do the rest - $10 per sample.
• Capillary separation only - you provided purified sequencing products, we do the rest - $3 per sample.

There is a 16-sample minimum. If you submit fewer than 16 samples they will not be processed until we have obtained additional samples from other clients.

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Genotyping Service

We offer the following option:

• Full service - you supply the internal size standard, you prepare the plate, and we take care of the rest - $3 per sample

There is a 16-sample minimum. If you submit fewer than 16 samples they will not be processed until we have obtained additional samples from other clients.

Email us on听dnaseq@exchange.canterbury.ac.nz听to make a booking. Once you have a booking, download the relevant request form and email it to us, as well as including a hard copy with your samples. 听Turnaround time is usually 24 hours.

• Sequencing internal request form (Excel 22KB)
• Genotyping internal request form (Excel, 22KB)

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Results

Turnaround is usually 24 hours. 听All results are emailed except for internal undergraduate and postgraduate students, who will receive their results on a flash drive.

We operate an archiving service. Past results can be requested, but there is a charge for this service.

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For all enquiries please contact:

Professor Tammy Steeves

Technical Officer Claire Galilee

The New 九州影院Analytical Ultracentrifugation Centre (NZAUC) is the only centre of its kind in New Zealand. The analytical ultracentrifuge allows researchers to probe the molecular mass of biomolecules in solution and to characterise interactions between different biomolecules (e.g. protein-DNA interactions).

Developments in instrumentation and data analysis have greatly increased the potential of the facility to deal with complicated systems, including inorganic crystalline nanoparticles and interactions within cell lysates and food, and to provide information complementary to other techniques, such as surface plasmon resonance (SPR).

The AUC is accessible to outside organisations on a contract or collaborative basis. For more information contact听biomolecular@canterbury.ac.nz.

An automated platform used for size and quantitation analysis of proteins, DNA and RNA. It works through microfluidic separation that produces size and electropherogram results for quantitative analysis from pure samples to cell lysates.

Within BIC we have a range of biomolecule purification equipment, including various AKTA systems (Pure, Basic and Express systems) and associated columns and media for any type of purification. 听We also have detection systems for absorbance and fluorescence.

Circular Dichroism spectroscopy allows the determination of protein secondary and tertiary structure. 听It can also be used to evaluate DNA quadruplex structure. 听It is often used to determine the chemical or temperature stability of biomolecules.

A confocal microscope detects the fluorescence emitted when the specimen is scanned by a laser, resulting in high-resolution images and the ability to directly observe dynamic processes in live cells. Cell components can be precisely localised, and accurate three-dimensional information is easily obtained. The behaviour and interactions of molecules can be studied.

Our inverted Leica TCS SP5 confocal microscope is equipped with a full range of lasers from 405 nm to 633 nm, as well as incubation and heating/cooling chambers.

The confocal microscope is accessible to outside organisations on a contract or collaborative basis.

Determine protein folding, domain structure and the effect of solution conditions, mutations, ligand binding and chemical labelling on protein conformation.

A dynamic light scattering system for particle and molecular weight measurements; analysing protein charge and iso-electric point through pH titration; assessing protein aggregation, oligomerisation state and protein-protein binding in a range of solvents; measurements of colloid and emulsion solutions.

This system is able to detect the presence of tagged protein or analyse binding kinetics. Binding events can be viewed in real time, and听 kinetic rates measured for association, dissociation and affinity. Assays require only 4 ul of sample, and can be performed in complex mixtures, or even crude solutions.

This analytical facility allows the high-resolution analysis and separation of anions. The detector system used is electrochemical (pulsed amperometric detection, PAD, sometimes also called pulsed electrochemical detection, PED), and is extremely sensitive; significantly more so than typical HPLC detectors.

A wide variety of different columns are available for use with the system, which allow a very broad range of analytical applications.

In addition to anions, as the eluent used is sodium hydroxide, weakly acidic organic compounds, such as reducing sugars are also very amenable to analysis. The ICS system is capable of mono-, oligo- and polysaccharide analysis and profiling, and finds applications in disciplines as disparate as Glycobiology and Food Science.

This instrument is accessible to outside organisations. It requires the purchase of specific columns and eluent.听

The interaction between molecules is often accompanied by a change in thermal energy. 听ITC quantifies this change allowing the precise determination of the thermodynamics of a biomolecular interaction. It is useful for protein-protein, protein-peptide, protein-ligand and protein-small molecule interactions.

MicroScale Thermophoresis听(MST)听is an immobilization-free technology for measuring biomolecular interactions. The MST instrument detects the motion of fluorescent molecules along a microscopic temperature gradient, which reflects changes in the molecular hydration shell, charge, or size. Since one or all of these parameters will change with virtually every binding event, a wide range of biomolecules can be measured, from ions and small molecule fragments to large macromolecular complexes, in small volumes (~20 渭l), in a wide range of standard buffers and complex mixtures such as liposomes, detergent, serum, and cell lysates.

The听NanoLab听contains facilities for semiconductor material processing, nanofabrication, and sensor and microfluidic device development.

The equipment covers most aspects of semiconductor device fabrication, from materials growth and characterisation to device packaging. The principal tools are:

• Atomic Force Microscope (Digital Instruments Dimension 3100)
• Deep Reactive Ion Etching (Oxford PlasmaPro 100 Cobra)
• Electron Beam Lithography (Raith 150)
• Interference Lithography
• Mask Writing (Heidelberg uPG101)
• Micromilling (CNC Mini- Mill/GX)
• Nanoimprint Lithography (EVG)
• Optical Lithography (Suss MA-6)
• Optical Microscopy (Olympus BX30 with digital image capture)
• Plasma Ashing (Tergeo plasma cleaner)
• Semiconductor Device Characterisation (HP 4155A Parameter Analyser)
• Surface Profilometer (Profilm3D)
• Thin Film Deposition (Edwards AUTO 500, Temescal FC-1800 and Mist CVD)
• Wire Bonding (Kulicke & Soffa 4500)

The facility is accessible to external organisations on a contract or collaborative basis.

Equipped with a Fluorescence and Florescence anisotropy detector and heating to 60 degrees.

Set up protein crystallisation trials easily and effectively. 听The drop sizes are usually 200鈥�400 nL and the usual setup is a 96 well plate.

Combines a thermal cycler and optical reaction module for singleplex and multiplex detection of fluorophores. The system features thermal gradient functionality and automation capabilities. In addition to real-time polymerase chain reactions (PCR), the instrument also offers rapid and simple protein stability screens by thermal melts.

Scattering of visible light can be used to determine the size and overall dimensions of macromolecules in solution. Absolute molecular weight can then be calculated directly from the light scatter, but this requires accurate measurement of protein concentration. To achieve this the Viscotek has detectors for refractive index and UV. Additionally, the Viscotek has a viscometer, which allows determination of molecular size, conformation, and structure.

A protein interaction array system that enables label-free quantitative analysis of biomolecular interactions in real time using surface plasmon resonance (SPR) technology. The interactions are monitored over time by flowing an analyte in a microfluidic channel over a ligand immobilized on a sensor chip and detecting the binding of the analyte to the ligand by measuring changes in the propagation of electromagnetic waves at the sensor surface.

Can analyse up to 36 different protein, peptide, DNA and small molecule interactions in a single run.

Utilised to monitor the speed of biomolecular reactions. Equipped with multicell block and temperature control.

The Bruker 600 MHz with a cooled sampler allows for automated measurement of a large number of biological samples. This is an optimal set up for the measurement of clinical samples and is currently the only one of its kind in Aotearoa New Zealand. Our instrument also allows for the analysis of large biological molecules sich as proteins, nucleic acids and carbohydrates.